evaluation of dot-elisa method using excretory-secretary antigens of fasciola hepatica in laboratory diagnosis of human fasciolosis

fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. in this study, dot-elisa, one of the simplest and the most sensitive tests in this regard, was evaluated using excretory-secretory antigens of fasciola hepatica to diagnose human fasciolosis three groups consisting of patients infected with fasciolosis (n= 95), patients with other parasitic diseases (n= 37) and healthy individuals (n= 40), were implicated in the test. all collected sera were tested by dot-elisa using excretory-secretory antigens. optimal criteria were detected as 1.5 µg of antigen per dot, serum dilution of 1:320, and anti human igg conjugate dilution of 1:500. the sensitivity, specificity, positive and negative predictive values were 96.8%, 96.1%, 96.8% and 96.1%, respectively. in conclusion, dot-elisa using excretory-secretory antigens could be regarded as a cheap, rapid, antigen and serum conservative diagnostic method in diagnosing fasciolosis.